Vaccinia Virus and Poxvirology

Working Safely with Vaccinia Virus: Laboratory Technique and the Role of Vaccinia Vaccination Stuart N. Isaacs Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers Maria M. Lorenzo, Inmaculada Galindo, and Rafael Blasco Construction of Recombinant Vaccinia Virus: Cloning into the Thymidine Kinase Locus Chelsea M. Byrd and Dennis E. Hruby Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses Mohamed Ragaa Mohamed and Edward G. Niles Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA, Xiao-Dan Yao and David H. Evans Construction of cDNA Libraries in Vaccinia Virus Ernest S. Smith, Shuying Shi, and Maurice Zauderer Construction and Isolation of Recombinant MVA Caroline Staib, Ingo Drexler, and Gerd Sutter Growing Poxviruses and Determining Virus Titer Girish J. Kotwal and Melissa-Rose Abrahams Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR Rachel L. Roper Orthopoxvirus Diagnostics Hermann Meyer, Inger K. Damon, and Joseph J. Esposito An In Vitro Transcription System for Studying Vaccinia Virus Early Genes Steven S. Broyles and Marcia Kremer An In Vitro Transcription System for Studying Vaccinia Virus Late Genes Cynthia F. Wright Studying Vaccinia Virus RNA Processing In Vitro Paul D. Gershon Methods for Analysis of Poxvirus DNA Replication Paula Traktman and Kathleen Boyle Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus Mansun Law and Geoffrey L. Smith Pox, Dyes, and Videotape: Making Movies of GFP-Labeled Vaccinia Virus Brian M. Ward Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance Bruce T. Seet and Grant McFadden Monitoring of Human Immunological Responses to Vaccinia Virus Richard Harrop, Matthew G. Ryan, Hana Golding, Irina Redchenko, and Miles W. Carroll Vaccinia Virus as a Tool for Immunologic Studies Nia Tatsis, Gomathinayagam Sinnathamby, and Laurence C. Eisenlohr Mouse Models for Studying Orthopoxvirus Respiratory Infections Jill Schriewer, R. Mark L. Buller, and Gelita Owens Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors Katharine N. Bossart and Christopher C. Broder Use of Dual-Recombinant Vaccinia Virus Vectors to Assay Viral Glycoprotein-Mediated Fusion with Transfection-Resistant Primary Cell Targets Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman Poxvirus Bioinformatics Chris Upton Preparation and Use of Molluscum Contagiosum Virus from Human Tissue Biopsy Specimens Nadja V. Melquiot and Joachim J. Bugert Index