Structure and Function of Membrane Proteins

Expression and Purification of Human Mitochondrial Intramembrane Protease PARL.- Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies.- Biochemical Characterization of GPCR–G Protein Complex Formation.- Electrophysiological Approaches for the Study of Ion Channel Function.- Isothermal Titration Calorimetry of Membrane Proteins.- Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level.- Structure Determination of Membrane Proteins Using X-Ray Crystallography.- Studying Membrane Protein Structures by MicroED.- Single-Particle Cryo-EM of Membrane Proteins.- Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy.- NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel.- Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering.- Preparing MembraneProteins for Simulation Using CHARMM-GUI.- Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide.- Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins.- Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions.- Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations.- Large Scale Molecular Dynamics Simulations of Cellular Compartments.