Effector-Triggered Immunity

A Brief Introduction to Effector-Triggered Immunity .- Lambda Red Recombineering in Shigella flexneri.- Using Genomic Deletion Mutants to Investigate Effector-Triggered Immunity during Legionella pneumophila Infection.- Methods of Bacterial Membrane Vesicle Production, Purification, Quantification, and Examination of their Immunogenic Functions.- Gene Gun-Mediated Transient Gene Expression for Functional Studies in Plant Immunity.- Host Induced Gene Silencing using BPMV on Soybean to Study Genes in the Soybean Rust Fungus Phakopsora pachyrhizi.- Virus-Mediated Protein Overexpression (VOX) in Monocots to Identify and Functionally Characterize Fungal Effectors.- Time-Resolved Fluorescence Microscopy Screens on Host Protein Subversion during Bacterial Cell Invasion.- Assaying RIPK2 Activation by Complex Formation.- Identification of Novel Endogenous NOD Ligands: Quantitative Analysis of Binding Affinities of NOD1 or NOD2 with Sphingosine-1-Phosphate using Microscale Thermophoresis.- A Surface Plasmon Resonance-Based Strategy to Characterize Interactions of NLR Proteins with Associated Factors.- Functional Assessment of Disease-Associated Pyrin Variants.- An Assay for the Seeding of Homotypic Pyrin Domain Filament Transitions.- Detection of Gasdermin Activation and Lytic Cell Death during Pyroptosis and Apoptosis.- Visualizing Effector Triggered Immunity in Response to Pore-Forming Toxins by Live-Cell Imaging.- Non-Apoptotic Cell Death Control of Neutrophil Extracellular Trap Formation.- Analysis of Bacteria-Triggered Inflammasome-Activation in Neutrophils by Immunoblot.- Quantifying Regulated Mitochondrial Fission in Macrophages.- Live Cell Imaging of T Cell Pyroptosis.