Embryonic Stem Cell Protocols

Dissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens.- Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions.- Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish.- Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells.- High-Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model.- Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications.- Feeder Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol.- ChIP-qPCR for Polycomb Group Proteins during Neuronal Differentiation of Human Pluripotent Stem Cells.- Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids.- Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs).- Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.- Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo.- Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells.- Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions.- A Simple, Rapid, and Cost-effective Method for Loss-of-Function Assays in Pluripotent Cells.- Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation.- A Modified SMART-Seq Method for Single Cell Transcriptomic Analysis of Embryoid Body Differentiation.- Osteogenic Differentiation from Mouse Embryonic Stem Cells.- Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation.- Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions.- Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages.- Accessing the Human Pluripotent Stem Cell Translatome byPolysome Profiling.- Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors.- Proteomic Analysis of Human Neural Stem Cells Differentiation by SWATH-MS.