Germline Development in the Zebrafish

Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish.- The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins.- Computer-Assisted Sperm Analysis to Test Environmental Toxicants.- Cryopreservation and Transplantation of Spermatogonial Stem Cells.- Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos.- Chemical Genetics: Manipulating the Germline with Small Molecules.- In vitro Induction of Teleost PGCs.- Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization.- Cryopreservation of Pooled Sperm Samples.- Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery.- In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads.- Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries.- Detection of the Polar Body after Fertilization.- Labelling the Micropylar Cell in Zebrafish Whole-mount and Cryo-sectioned Follicles.- Visualisation of Transcriptional Activity in Early Zebrafish Primordial Germ Cells.- Experimental Methodologies to Visualise Early Cell Biology in Zebrafish Embryos.- Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy.- Methods for Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish Embryo.- Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining.- Histological Analysis of Gonads in Zebrafish.- Manipulating and Visualizing the Germline with Transgenic Lines.-  Liquid Chromatography and Tandem Mass Spectrometry in Label Free Protein Quantification of Zebrafish (Danio rerio) Eggs.- In vivo Protein Lifetime Measurements across Multiple Organs in the Zebrafish.- In vivo Imaging of Protein Interactions in the Germ Plasm with Bimolecular Fluorescent Complementation.- The Zygotic Genome Activation: Critical Prelude to the Most Important Time of Your Life.- Identification of RNA-binding Protein Landscapes Across Zebrafish Embryonic Transcriptome via iCLIP Approach.- Tethered Function Assay to Study RNA Regulatory Proteins in Zebrafish Embryos.- -Massively Parallel Analysis of Regulatory RNA Sequences.- Exploring Translational Control of Maternal mRNAs in Zebrafish.