Cancer Cell Biology

Overview of the maintenance of authentic cancer cell lines.- Culturing suspension cancer cell lines.- Culture of Adherent Cancer Cell Lines.- Breast Cancer Xenograft Murine Models.- Processing and cryopreservation of blood, cancer tissues and cancer cells for viable biobanking.- Adipocyte-breast cancer cell co-culture in transwells.- A 3-dimensional co-culture method for studying interactions between adipocytes, extracellular matrix, and cancer cells.- Modelling Collective Invasion and Single-Cell Mesenchymal Invasion in Three-Dimensional Matrigel-Collagen I Cultures.- CellTrace™ Violet Flow Cytometric Assay to Assess Cell Proliferation.- Enzyme-Linked Immunosorbent Assay (ELISA).- Flow cytometry analysis to detect lapatinib-induced modulation of constitutive and IFN-g induced HLA class I expression in HER2-positive breast cancer cells.- An end-to-end workflow for interrogating tumour infiltrating myeloid cells using mass cytometry.- Manual Immunofluorescence of Formalin-Fixed Paraffin-Embedded Human Tumour Tissues.- Western blot analysis of lapatinib-mediated inhibition of the epidermal growth factor receptor 2 (HER2) pathway in breast cancer cells.- Evaluating cell membrane localization and intracellular transport of proteins by biotinylation.- Multiplexed quantitative proteomic profiling of cancer cells and tissues using isobaric labeling-based tags.- The use of Seahorse XF assays to interrogate real-time energy metabolism in cancer cell lines.- Using CRISPR-Cas9 to dissect cancer mutations in cell Iines.- Single-cell RNA sequencing analysis using Fluidigm C1 platform for characterization of heterogeneous transcriptomes.- Sequences to Differences in Gene Expression: Analysis of RNA-Seq Data.- Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) for gene expression analyses.- Peptide-affinity isolation of extracellular vesicles and cell-free DNA from human plasma.- Characterizing Extracellularvesicles using Nanoparticle Tracking Analysis.