Heterologous Expression of Membrane Proteins

Solubilization and Purification of a5b1 Integrin from Rat Liver for Reconstitution into Nanodiscs.- Membrane Proteins Production in Escherichia coli: Protocols and Rules.- Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly.- Heterologous Expression of Membrane Proteins in E. coli.- Overexpression of the ABC Transporter BmrA within Intracellular Caveolæ in Escherichia coli.- Production of Membrane Proteins in Pseudomonas stutzeri.- Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae.- Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae.- Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p.- Membrane Protein Production in the Yeast, P. pastoris.- Production and Preparation of Isotopically-Labelled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses.- Membrane Protein Production in Insect Cells.- Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies.- Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies.- The Heterologous Expression and Purification of GPCRs.- Expression and Purification of the Human Thyroid-Stimulating Hormone Receptor.- Optimization of Recombinant GPCR Proteins for Biophysical and Structural Studies Using Virus-Like Particles.- Peptide Tags and Domains for Expression and Detection of Mammalian Membrane Proteins at the Cell Surface.- Tailor the Purification and Analysis of Membrane Proteins with Modular Detergents.- Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers.- Detergent-Free Membrane Protein Purification Using SMA Polymer.- Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies.- Cell-Free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs.- Microscale Thermophoresis to Evaluate the Functionality of Heterologously Overexpressed Membrane Proteins in Membrane Preparations.