Methods in Stem Cell Biology - Part B

Dr Ilio Vitale is an esteemed oncologist. He is a member of the University of Rome and associated to the Regina Elena National Cancer Institute based in Italy. Gwenola Manic received her Ph.D. from the University of Paris Sud/Paris XI (France) in 2012 studying the impact of the design of HIV-1-derived vectors and DNA repair machinery on lentiviral expression. At present she is working as a postdoctoral fellow at the Department of Biology at the Università degli Studi di Roma “Tor Vergata” in Rome (Italy) on the contribution of chromosomal instability in tumorigenesis and the therapeutic targeting of cancer stem cells. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. Multiple Gene Knockdown Strategies for Investigating the Properties of Human Leukemia Stem Cells and Exploring New Therapies Xiaoyan Jiang 2. Assessment of CD133, EpCAM, and CD44 in human and murine cancer cell by real-time qPCR Guillermo Mazzolini 3. Reverse Phase Protein Array in cancer stem cells Michele Signore 4. Isolation of cancer stem cells from squamous cell carcinoma Sanjeev K. Centre (TMC), Advanced Centre for Treatment and Research and Education in Cancer (ACTREC), 5. Immunoblotting-assisted assessment of JAK2 signaling in leukemic stem cells Niccolò Bartalucci 6. Isolation of cancer stem cells from based on aldehyde dehydrogenase activity Paola Marcato 7. Generation of neuronal/glial mixed cultures from human induced pluripotent stem cells (hiPSCs) Angela Gritti 8. Immunohistochemistry-based analysis of EMT markers in mammary tumors Diwakar Pattabiraman 9. Immunofluorescence staining of colorectal cancer patient-derived organoids Joseph L. Regan 10. Isolation of murine bone marrow hematopoietic stem and progenitor cell populations via flow cytometry Michael A. Rieger 11. In vitro and in vivo limiting dilution assay of hepatocellular carcinoma cells Bin Wang Sr., Yingying Lai and Xi Zheng 12. Methods to isolate adipose tissue-derived stem cells Barbara Zavan, Paolo Pinton, Leticia Ferroni, Chiara Gardin and Francesco De Francesco 13. In vivo assessment of the tumorigenic and metastatic potential of the cancer stem cell fraction in breast cancer cells Antonella Sistigu