Marine Genomics

Mitochondrial Genome of Non-Model Marine Metazoans by Next-Generation Sequencing (NGS).- Genome-Wide DNA Methylation Protocol for Epigenetics Studies.- Transcriptome Mining to Identify Genes of Interest: From Local Databases to Phylogenetic Inference.- Detecting Structural Variants and Associated Gene Presence-Absence Variation Phenomena in the Genomes of Marine Organisms.- From Sequences to Enzymes: Comparative Genomics to Study Evolutionary Conserved Protein Functions in Marine Microbes.- VenomFlow: An Automated Bioinformatic Pipeline for Identification of Disulfide-Rich Peptides from Venom Arsenals.- Population Genomics Analysis with RAD, Reprised: Stacks 2.- A Metabarcoding Protocol to Analyze Coastal Planktic Communities Collected by Desalination Plant Filters: From Sampling to Bioinformatic Exploratory Analyses.- Barcoding of Antarctic Marine Invertebrates: From Field Sampling to Lab Procedures.- DNA Barcoding Procedures for Taxonomical and Phylogenetic Studies in Marine Animals:Porifera as Case Study.- Environmental DNA from Marine Waters and Substrates: Protocols for Sampling and eDNA Extraction.- Metataxonomic Analysis of Bacterial Diversity Associated with Marine Organisms.- From Sequences to Enzymes: Heterologous Expression of Genes from Marine Microbes.- Expression of Recombinant Cold-Adapted (Hemo)Globins from Marine Bacteria.- Isolation of UV-Resistant Marine Bacteria by UV-C Assays.- Fractionation Protocol of Marine Metabolites.- Detection and Quantification of Small Non-Coding RNAs in Marine Diatoms.- Optimized Proteolistic Protocol for the Delivery of the Cas9 Protein in Phaeodactylum tricornutum.- Production of a Chimeric Mouse-Fish Monoclonal Antibody by the CRISPR/Cas9 Technology.- Identification, Characterization, and Expression Analysis of Immunoglobulin Genes from Antarctic Fish by PCR Methods.- Physical Mapping of Repeated Sequences on Fish Chromosomes by Fluorescence In Situ Hybridization (FISH).- Functional Genomics of Fish Erythrocytes.- Stain-Free Approach for Western Blot Analysis of Zebrafish Embryos.- Proteomics of Fish White Muscle and Western Blotting to Detect Putative Allergens.- In Vitro Assays for the Bifunctional Acylpeptide Hydrolase (APEH) Enzyme from Antarctic Fish.