Proteomic Profiling

How Modern Mass Spectrometry Can Solve Ancient Questions: A multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi.- Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis.- Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis.- Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry.- Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation.- Phos-Tag Fluorescent Gel Staining for the Quantitative Detection of His- and Asp-Phosphorylated Proteins.- Polyubiquitin Profile in Down Syndrome and Alzheimer Disease Brain.- Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing.- A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes.- Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma.- Isolation of Extracellular Vesicles for Proteomic Profiling.- Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses.- Flow Cytometry as an Important Tool in Proteomic Profiling.- Improved Immunoprecipitation to Mass Spectrometry Method for Enrichment of Low-Abundant Protein Targets.- Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.- Bead-Based Multiplex Immunoassays: Procedures, Tips, and Tricks.- Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma.- Recombinant Anti-Idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development.- cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection.- Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions.- Profiling Protein-DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis.- Biotin Proximity Labeling for Protein-Protein Interaction Discovery: The BioID Method.- Studying OTUD6B-OTUB1 Protein-Protein Interaction by Low-Throughput GFP-Trap Assays and High-Throughput AlphaScreen Assays.- Thermal Shift Assay for Exploring Interactions between Fatty Acid-Binding Protein and Inhibitors.- Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses.- Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling.- Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an In Vitro Exercise Model.- Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Advantages of Stain-Free Technology.- Technical Considerations for Contemporary Western Blot Techniques.- Simple Western: Bringing the Western Blot into the 21st Century.- Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests.- Sample Preparation of Secreted Mammalian Host Cell Proteins and their Characterization by Two-Dimensional Electrophoresis and Western Blotting.- Quantitative Proteomic Analysis Using Formalin-Fixed Paraffin-Embedded (FFPE) Human Cardiac Tissue.- Chloroplast Isolation and Enrichment of Low Abundant Proteins by Affinity Chromatography for Identification in Complex Proteomes.- Principles of Protein Labeling Techniques.- Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization.