TGF-Beta Signaling
Springer-Verlag New York Inc.
978-1-0716-2276-6 (ISBN)
Authoritative and up-to-date, TGF-Beta Signaling: Methods and Protocols serves as a vital resource for researchers seeking to move the TGF-β field into the quantitative realm.
Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot.- Fast Quantitation of TGF-β Signaling Using Adenoviral Reporter.- Complex Formation among TGF-b Receptors in Live Cell Membranes Measured by Patch-FRAP.- Branched Proximity Hybridization Assay for the Quantification of Nanoscale Protein-Protein Proximity.- Visualizing Dynamic Changes during TGF-β-Induced Epithelial to Mesenchymal Transition.- Establishment of Embryonic Zebrafish Xenograft Assays to Investigate TGF-β Family Signaling in Human Breast Cancer Progression.- Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling.- CRISPR-Based Screening in Three-Dimensional Organoid Cultures to Identify TGF-β Pathway Regulators.- Optogenetic Control of TGF-β Signaling.- Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-β Signaling in Mouse Embryonic Stem Cells.- Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network.- Discrete Logic Modeling of Cell Signaling Pathways.- Mining of Single-Cell Signaling Time-Series for Dynamic Phenotypes with Clustering.- Automated Classification of Cellular Phenotypes Using Machine Learning in Cellprofiler and CellProfiler Analyst.- Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs.
Erscheinungsdatum | 06.04.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2488 |
Zusatzinfo | 45 Illustrations, color; 4 Illustrations, black and white; X, 239 p. 49 illus., 45 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Sachbuch/Ratgeber ► Sport |
Medizin / Pharmazie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik | |
Schlagworte | Cell signaling pathways • computational methods • Quantitative experiments • TGF-β signaling • Transforming Growth Factor beta |
ISBN-10 | 1-0716-2276-5 / 1071622765 |
ISBN-13 | 978-1-0716-2276-6 / 9781071622766 |
Zustand | Neuware |
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