Circadian Regulation

Sleep under Preindustrial Conditions: What We Can Learn from It.- The structure-based molecular-docking screen against core clock proteins to identify small molecules to modulate the circadian clock.- Analysis of complex circadian time series data using wavelets.- Mathematical Modeling in Circadian Rhythmicity.- Bioinformatics and Systems Biology of Circadian Rhythms: BIO CYCLE and CircadiOmics.- Cell-based Phenotypic Screens to Discover Circadian Clock Modulating Compounds.- Methods for Assessing Circadian Rhythms and Cell Cycle in Intestinal Enteroids.- Using ALLIGATORs to capture circadian bioluminescence.- Studying circadian clock entrainment by hormonal signals.- In vitro assays for measuring intercellular coupling among peripheral circadian oscillators.- Circadian control of transcriptional and metabolic rhythms in primary hepatocytes .- Electrophysiology of the Suprachiasmatic Nucleus: Single-Unit Recording.- AnatomicalMethods to Study the Suprachiasmatic Nucleus.- Circadian analysis of rodent locomotor activity in home cages.- Recording of diurnal gene expression in peripheral organs of mice using the RT-Biolumicorder.- Isolation and sorting of epidermal interfollicular stem cells for the study of circadian rhythms.- Detecting circadian rhythms in human red blood cells by dielectrophoresis.- Measuring circadian neutrophil infiltration in tissues by paired whole-mount tissue-clearing and flow cytometry.- In vivo imaging of circadian NET formation during lung injury by 4-dimensional intravital microscopy.- Real-time measurement of energy metabolism over circadian time using indirect calorimetry-enabled metabolic cages.- Untargeted and Targeted Circadian Metabolomics Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Flow Injection-Electrospray Ionization-Tandem Mass Spectrometry (FIA-ESI-MS/MS).- Time Restricted Feeding and Caloric Restriction - two feeding regimen at the crossroad of metabolic and circadian regulation. -Chromatin Immunoprecipitation and Circadian Rhythms.- Fluorescent reporters for studying circadian rhythms in Drosophila melanogaster.- Visualization of mutant aggregates from clock neurons by agarose gel electrophoresis (AGERA) in Drosophila melanogaster.- Methods for delivery of dsRNAi against canonical clock genes and immunocytodetection of clock proteins in crustacea.- In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer.