Carotenoids: Carotenoid and Apocarotenoid Analysis

As a Ph.D student, Eleanore Wurtzel innovated gene tagging and isolated the first genes for two-component signaling in bacteria, laying the foundation for study of signaling mechanisms found throughout nature, including plants. With an NSF postdoctoral fellowship, Dr. Wurtzel boldly changed fields from bacterial membrane biochemistry to plant biology, when maize was the only model system. She established some of the first experiments on plant chromatin structure as an NSF Plant Biology postdoctoral fellow at Brookhaven National Laboratory. She then joined Cold Spring Harbor Laboratory and began research on maize carotenoid biosynthesis, then a poorly studied area. Dr. Wurtzel next joined the Biological Sciences Department at Lehman College, City University of New York, where she is currently a Full Professor and on the faculty of the CUNY Biology and Biochemistry PhD programs. Eleanore Wurtzel has made fundamental and longstanding contributions to the field of plant carotenoid biosynthesis, plant biochemistry, and plant metabolic engineering which are enabling improvement of crops for sustainable solutions to global vitamin A deficiency affecting the health and mortality of 250 million children worldwide. Dr. Wurtzel is grateful to the many students, postdocs, and visiting scientists who have contributed to her laboratory’s research for which she has been recognized as a Fellow of AAAS, Fellow of ASPB, and most recently as a Fellow of the International Carotenoid Society. Dr. Wurtzel serves as a Monitoring Editor of Plant Physiology. Dr. Wurtzel has also been a long-standing elected member of the Gordon Research Conferences (GRC) Board of Trustees. She has been instrumental at GRC in developing and contributing to programs for women in science. She also founded and chaired the first GRC on Plant Metabolic Engineering and founded the GRC seminar for early career scientists for both the GRC Plant Metabolic Engineering community and the GRC Carotenoids community.

1. Getting to know carotenoids George Britton 2. Laser capture of tissues for micro-scale carotenoid analyses James Giovannoni 3. Metabolic engineering of carotenoids: procedures for metabolomic characterization Paul David Fraser 4. LC-MS analysis of intracellular metabolites for precursors to the carotenoid pathway Claudia Vickers 5. Use of E. coli to produce carotenoid standards Norihiko Misawa, Miho Takemura and Takashi Maoka 6. HPLC analysis of carotenoids from Bacteria Gerhard Sandmann 7. Purification and development of standards for carotenoid quantification in plant tissues Christopher Ian Cazzonelli, Jwalit Nayak, Yagiz Alagoz and Sidra Anwar 8. Ultra-High Performance Liquid Chromatography-Mass Spectrometry Analysis of Plant Apocarotenoids Salim Al Babili, Jianing Mi, Yagiz Alagoz, Juan Camilo Moreno, Kit Xi Liew, Aparna Balakrishna and Xiongjie Zheng 9. Detection and analysis of novel and known volatile plant apocarotenoids Barry James Pogson, Ryan McQuinn, Julie Leroux and Thy Thuc Truong 10. Carotenoid extraction, detection, and analysis in citrus Xiuxin Deng, Kaijie Zhu, Xiongjie Zheng, Yun Liu, Hongyan Chen, Yingzi Zhang and Junli Ye 11. Strategies For The Separation And Tentative Identification Of Geometrical (Cis/Trans, Z/E) Isomers Of Carotenoids Antonio J. Meléndez-Martínez 12. Use of stable isotopes to study bioconversion and bioefficacy of pro-vitamin A carotenoids Georg Lietz 13. Carotenoid extraction and analysis of blood plasma/serum Jessica L. Cooperstone and Maria Sholola 14. Methods for analyzing carotenoids in bird tissues Kevin McGraw and Matthew Toomey 15. Analysis of plant-derived carotenoids in camouflaging stick insects Royce Cumming, Kaijie Zhu, Eleanore T. Wurtzel and Jeffry Petracca