Cotton Breeding and Biotechnology

Dr. Zulqurnain Khan is an Assistant Professor at the Institute of Plant Breeding and Biotechnology (IPBB), MNS University of Agriculture, Multan (MNSUAM), Pakistan. Dr. Khan earned his PhD in Biotechnology from the University of Agriculture, Faisalabad (UAF), Pakistan, in 2017. He is the first PhD candidate from Pakistan to have a thesis on genome editing. He has been working in the field of genome editing since 2012. His research focuses on using genome-editing tools (TALEs, TALENs, Cas9, dCas9, and multiplexed CRISPR/Cas9) for resistance against begomoviruses using model plants such as Arabidopsis and Nicotiana benthamiana. He has developed resistance in cotton against CLCuD using multiplex CRISPR/Cas system. He is also using CRISPR/Cas9 technology for the genetic improvement in cereal crops for abiotic and biotic stress resistance. He has published one book chapter, two review articles, and three research articles in international peerreviewed journals. Dr. Khan received an IRSIP fellowship from Higher Education Commission (HEC), Pakistan, during his PhD and worked as a visiting researcher with Prof. Caixia Gao at the Institute of Genetics and Developmental Biology (IGDB), Chinese Academy of Sciences (CAS), Beijing, China. Dr. Khan has been engaged in teaching various courses in the field of biotechnology, breeding, molecular biology, genetics, and genome editing since 2018. Dr. Khan has recently edited two books for Springer Nature entitled “CRISPR Crops: The Future of Food Security” and “The CRISPR/Cas Tool Kit for Genome Editing”. Professor Zulfiqar Ali is a Professor at the Institute of Plant Breeding and Biotechnology and Principal Officer in the Office of Research Innovation and Commercialization (ORIC), MNS University of Agriculture, Multan (MNSUAM), Pakistan. Prof. Ali completed his PhD from the University of Agriculture, Faisalabad, Pakistan, and postdoctoral studies from Sydney University Plant Breeding Institute (2007–2008) and Jiangsu Academy of Agricultural Sciences Nanjing (2009–2011). He has published more than 80 books/chapters/research papers in highly popular journals like Plant Breeding, Nature’s Scientific Report, Rice, Field Crop Research, PLOS One, and has been serving as the section editor of Pakistan Journal of Agricultural Sciences. Prof. Ali has successfully completed various research projects. Currently, he is running six research projects funded by national and international funding agencies including HEC, PARB, ADP, USAID, DFID, ACIAR, etc. He has been granted awards, including the Gold Medal 2017 in Biotechnology from the Pakistan Academy of Sciences. Recently, Dr. Ali has also been awarded with the “National Level HEC Best Teacher Award 2021” by Higher Education Commission (HEC), Pakistan. For over the past 21 years, Prof. Ali has been engaged in the fields of plant biotechnology, breeding, genetics, and molecular biology. His research focuses on climate-smart breeding using functional genomics tools. Professor Asif Ali Khan is the Vice-Chancellor, MNS University of Agriculture, Multan (MNSUAM), Pakistan, since 2016. He did two postdocs from UC-Davis, USA, and Liverpool, UK. During his professional career, he has served at many key posts at the University of Agriculture, Faisalabad, the Director Office of Research Innovation and Commercialization (ORIC); Director, Business Incubation Centre (BIC); Director, External Linkages. Prof. Khan has been teaching plant breeding and genetics for the past 32 years. Prof. Khan has completed research projects funded by national and international agencies. Prof. Khan has published more than 140 articles in national and international impact factor journals. Prof. Khan has also edited and authored various books and book chapters. Prof. Khan received several fellowships and awards. Prof. Khan is a renowned geneticist and has completed more than ten research projects awarded by different national and international agencies. Over the past 32 years, Prof. Khan has been engaged in the field of genetics, evolution, functional genomics, plant biotechnology, and plant breeding.

Contents

Foreword...........................................................................................................................................vii

Editors................................................................................................................................................ix

List of Contributors............................................................................................................................xi

Chapter 1 Role of Breeding and Biotechnology in Sustainable Cotton Production......................1

Zulqurnain Khan, Zulfiqar Ali and Asif Ali Khan

Chapter 2 Cotton Genetics and Genomics...................................................................................15

Muhammad Umar Iqbal, Sultan Habibullah Khan, Muhammad Salman Mubarik,

Aftab Ahmad, Muhammad Sajjad and Hafiz Muhammad Imran

Chapter 3 Conventional Breeding of Cotton................................................................................29

Farzana Ashraf, Nadia Iqbal, Wajid Nazeer, Zia Ullah Zia, Asif Ali Khan

and Saghir Ahmad

Chapter 4 Molecular Cotton Breeding.........................................................................................47

Zulqurnain Khan, Zulfiqar Ali and Asif Ali Khan

Chapter 5 Gene Mapping in Cotton.............................................................................................69

Muhammad Abu Bakar Saddique, Zulfiqar Ali, Muhammad Ali Sher,

Babar Farid, Furqan Ahmad and Sarmad Frogh Arshad

Chapter 6 Functional Genomics in Cotton...................................................................................87

Furqan Ahmad, Akash Fatima, Plosha Khanum, Shoaib ur Rehman,

Muhammad Abu Bakar Saddique, Mahmood Alam Khan and Zulqurnain Khan

Chapter 7 Cotton Transformation and Regeneration.................................................................101

Ummara Waheed, Sadia Shabir, Fatima Mazhar, Mamoona Rehman,

Hamza Ahmad Qureshi and Nadia Iqbal

Chapter 8 Breeding Cotton for Heat Tolerance..........................................................................113

Muhammad Kashif Riaz Khan, Fang Liu, Baohua Wang, Manzoor Hussain,

Allah Ditta, Zunaira Anwar and Aqsa Ijaz

Chapter 9 Breeding Cotton for Drought Tolerance....................................................................139

Muhammad Mahmood Ahmed, Muhammad Waseem Akram and Zulfiqar Ali

Chapter 10 Breeding Cotton for Salt Tolerance...........................................................................157

Zulfiqar Ali, Muhammad Tehseen Azhar, Muhammad Mahmood Ahmed,

Umar Akram and Abid Hussain

Chapter 11 Breeding Cotton for Cotton Leaf Curl Disease Resistance.......................................171

Judith K. Brown and Zulqurnain Khan

Chapter 12 Breeding Cotton for Insects/Pests Resistance...........................................................199

Hafiza Masooma Naseer Cheema, Asif Ali Khan, Muhammad Aakif Khan,

Muhammad Ans Pervez, Muhammad Zubair Ghouri, Aftab Ahmad

and Sultan Habibullah Khan

Chapter 13 Breeding Cotton for Herbicide Resistance................................................................233

Muhammad Zaffar Iqbal and Shahid Nazir

Chapter 14 Breeding Cotton for Fiber Traits...............................................................................245

Nadia Iqbal, Farzana Ashraf, Ummara Waheed and Saghir Ahmad

Chapter 15 Breeding Cotton for Value Added Traits...................................................................259

Sajid Majeed, Muhammad Salman Mubarik, Muhammad Umar Iqbal,

Xiongming Du and Muhammad Tehseen Azhar

Chapter 16 Genetically Modified Cotton: Boom or Dust............................................................271

Iqrar Ahmad Rana, Allah Bakhsh, Shakhnozakhon Tillaboeva, Qandeel-e-Arsh,

Shang Haihong and Muhammad Tehseen Azhar

Chapter 17 Cotton Seed System..................................................................................................291

Fawad Salman Shah, Muhammad Aslam Bhatti, Asif Ali Khan,

Irfan Ahmad Baig, Muhammad Hammad Nadeem Tahir, Furqan Ahmad

and Muhammad Amir Bakhtavar

Chapter 18 Cotton Breeding and Entrepreneurship: Challenges and Opportunities...................311

Mubashir Mehdi, Muhammad Haseeb Raza and Muhammad Talha Azeem

Chapter 19 Breeding Cotton for International Trade...................................................................323

Khalid Abdullah and Zahid Khan

Index .......................................................................................................................................339