Molecular Embryology

Part I. Mouse Embryo. Mouse Embryo as and Experimental System. Culture of Postimplantation Mouse Embryos. Organ Culture and the Analysis of Tissue Interactions. Treatment of Mice with Retinoids In Vivo and In Vitro and Skeletal Preparations. Cell Grafting and Labeling in Post-implantation Mouse Embryos. Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-cell Eggs. Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells. Gene Trapping in Mouse Embryonic Stem Cells. Production and Growth of Conditionally Immortal Cell Lines from the H-2KbtsA58 Transgenic Mouse. Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos. Application of lacZ Transgenic Mice to Cell Lineage Studies. Mouse Primordial Germ Cells: Isolation and In Vitro Culture. Part II. Chicken Embryo. The Avian Embryo: An Overview. Chicken Embryos: Incubation and Isolation. New Culture. Grafting of Hensen's Node. Grafting of Somites. Manipulating the Chick Embryo: Notochord Grafts. Grafting of Neural Tissue. Grafting of Apical Ridge and Polarizing Region. Tissue Recombinations in Collagen Gels. Chick-quail Chimera. Using Fluorescent Dyes for Fate-Mapping Lineage Analysis and Axon Tracing in the Chick Embryo. Part III. Amphibian Embryos. An Overview of Xenopus Development. Mesoderm Induction Assays. Experimental Methods for Analysis of Neural Induction in the Amphibian. A Methods for Generating Transgenic Frog Embryos. The Axolotl and Newt. Part IV. Zebrafish. The Zebrafish: An Overview of its Early Development. Small- Scale Marker-based Screening for Mutations in Zebrafish Development. Transgenic Zebrafish. Microinjection of DNA RNA and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function. Part V. Non-Vertebrate Chordates. Protochordates. Part VI. Retrovirus. Gene Transfer to the Rodent Embryo by Retroviral Vectors. Gene Transfer Using Replication-Competent Avian Retroviruses. Part VII. Molecular Techniques. Retinoids. Subtractive Hybridization and Construction of cDNA Libraries. Differential Display of Eukaryotic mRNA. RT-PCR in Embryos Using Degenerate Oligonucleotide Primers. Single-Cell RT-PCR cDNA Subtraction. In Situ Hybridization to RNA in Tissue Sections Using Radioactive Probes. In Situ Hybridization to RNA in Whole Embryos. Whole Mount In Situ Hybridization to Xenopus Embryos. Whole Mount In Situ Hybridization to Amphioxus. Non-radioactive In Situ Hybridization to Tissue Sections. Immunohistochemistry Using Polyester Wax. Immunohistochemistry to Whole Embryos. Whole Embryo Assays for Programmed Cell Death. Antisens Oligonucleotides. Protein Techniques: Immunoprecipitation In Vitro Kinase Assays and Western Blotting. Part. VIII. Microscopy and Photography. Microscopy and Photomicography Techniques. Index.