Molecular Diagnosis of Infectious Diseases

I. Recombinant Proteins and Peptides. Identification of Novel Diagnostic Markers by Differential Display Peng Liang Feilan Wang Weimin Zhu Robert P. O'Connell and Lidia Averboukh. Immunoprecipitation Kari Johansen and Lennart Svensson. RT-PCR Methods and Applications Paul D. Siebert. Quantitative RT-PCR Paul D. Siebert. Cloning cDNA Ends Using RACE Yue Zhang and Michael A. Frohman. A Method for the Rapid Identification of Epitopes and Other Functional Peptide Domains Robert C. Mierendorf Beth Hammer and Robert E. Novy. Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Finetuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis Ulla Wienhues Wolfgang Kraas Christoph Seidel Hans Georg Ihlenfeldt and G?nther Jung. Amplification of Genomic DNA by PCR Bruno Frey. PCR-Based Cloning and Subsequent Expression of Antigenic Proteins in Escherichia coli Udo Reischl. Application of TEV Protease in Protein Production Deborah A. Polayes T. Dawn Parks Stephen A. Johnston and William G. Dougherty. Stable Transfected Drosophila Schneider-2 Cells as a Novel Tool to Produce Recombinant Antigens for Diagnostic Therapeutic and Preventive Purposes Ludwig Deml and Ralf Wagner. Use of Recombinant Antigens as Diagnostic Reagents in Enzyme-Linked Immunosorbent Assays Alan Warnes Anthony R. Fooks and John R. Stephenson. Generation of Recombinant Baculovirus DNA in E. coli Using a Baculovirus Shuttle Vector Valentina C. Ciccarone Deborah A. Polayes and Verne A. Luckow. The SFV Gene Expression System Valentina C. Ciccarone Joel A. Jessee and Peter Liljestrm. Expression and Purification of Recombinant Proteins Using the pET System Robert C. Mierendorf Barbara B. Morris Beth Hammer and Robert E. Novy. Procedures for the Analysis and Purification of His-Tagged Proteins Richard E. Kneusel Joanne Crowe Melanie Wulbeck and Joachim Ribbe. Detection and Immobilization of Proteins Containing the 6xHis Tag Richard E. Kneusel Melanie Wulbeck and Joachim Ribbe. The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins Louis E. C. Leong. Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) Udo Reischl. Purification of Recombinant Proteins Having High Isoelectric Points Raffaele A. Calogero and Anna Aulicino. Immunoblotting Michael Kirschfink and Peter Terness. Diagnostic Application of a Multiple-Channel Immunoblot Apparatus Shaun D. Black. Immobilization of Immunoreactants on Solid Phases for ELISAs Richard Decker and David Leahy. Nonradioactive Labeling and Immunodetection of Recombinant Proteins Robert Oberfelder. Flow Cytometry Volker Daniel. Basic Problems of Serological Laboratory Diagnosis Walter Fierz. Molecular Diagnostics Resources on the Internet Kevin J. O'Donnell. II. Antibodies. Monoclonal Antibody-Based Immunoassays Peter Appleby and Udo Reischl. Biosensor-Based Methods in Clinical Diagnosis Franz Aberl and Conrad Kalinger. The Quartz Crystal Microbalance (QCM) as an Immunosensor Franz Aberl Conrad Kalinger and Hans Wolf. Biomolecular Interaction Analysis Strategies for Antibody and Antigen Characterization Uwe W. Roder and Francis Markey. Recent Developments in Antibody Engineering Melvyn Little Stefan D?bel Sergey Kipriyanov and Frank Breitling. Cloning and Expression of Single Chain Fragments (scFv) from Mouse and Rat Hybridomas Frank Breitling and Stefan D?bel. Production of a Human Antibody Library in the Phage-Display Vector pSEX81 Martin Welschof Melvyn Little and Heinz Drsam. Screening of Phage-Displayed Antibody Libraries Heinz Drsam Michael Braunagel Christian Kleist Daniel Moynet and Martin Welschof. Purification Characterization and Biotinylation of Single-Chain Antibodies Sergey M. Kipriyanov. Index.