Opioid Receptors

Overview of Genetic Analysis of Human Opioid Receptors.- Renilla luciferase Reporter Assay to Study 3’UTR-driven post-Transcriptional Regulation of OPRM1.- Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1.- Monitoring Opioid Receptors Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET).- Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-visual Arrestins.- Functionalization and Bioconjugation of Nanoruby for Long-term, Ultrasensitive Imaging of Μ-Opioid Receptors.-- Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues.- Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells.- Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-tolerant Mice.- GTPgS-autoradiography for Studies of Opioid Receptor Functionality.- Evaluating Opioid Mediated Adenylyl Cyclase Inhibition in Live Cells using a BRET-based Assay.- Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury.- Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV.- An In Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion.- Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat.- The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls and Future Perspectives of Biased Agonists at Opioid Receptors.- Mechanical Nociception in Mice and Rats: Measurement with Automated von Frey Equipment.- Evaluation of Murine Macrophage Cytokine Production after In Vivo Morphine Treatment.- Measurement of Macrophage Toll like Receptor 4 Expression After Morphine Treatment.- Conditioned Place Preference (CPP) in Rats: From Conditioning to Reinstatement Test.- Analysis of Opioid-SeekingBehavior through the Intravenous Self-administration Reinstatement Model in Rats.- Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors in Rats and Mice.- Early Life Stress as a Probe to Study the Opioid System in Developing Rodents.- Evaluation of m--opioid System Functionality in Mouse Pups:  Ultrasonic Vocalizations as an Index Of Infant Attachment.