Selenoprotein Structure and Function

Eranthie Weerapana is an Associate Professor of Chemistry at Boston College. She received her B.S. in Chemistry from Yale University, and her Ph.D. in Chemistry from MIT, where she worked with Professor Barbara Imperiali, investigating glycosyltransferases involved in N-linked glycosylation in the gram negative bacterium Campylobacter jejuni. She then performed postdoctoral studies at The Scripps Research Institute, La Jolla where she worked with Professor Benjamin F. Cravatt to develop chemical-proteomic methods to investigate reactive cysteines in complex proteomes. Her interdisciplinary research program focuses on applying mass-spectrometry methods to identify regulatory cysteine residues in the human proteome, and chemical biology approaches to develop covalent small-molecule modulators for cysteine-mediated protein activities.

1. Identification of Selenoprotein O substrates using a biotinylated ATP analog Anju Sreelatha 2. Selenium-encoded isotopic signature targeted profiling Chu Wang 3. Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reporters Dieter Söll 4. Preparation of selenoprotein S by chemical ligation Sharon Rozovsky 5. Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells Dohoon Kim 6. SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyond Yaoyang Zhang 7. Selenocysteine substitutions in thiyl radical enzymes Brandon Greene 8. Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strain Elias S.J. Arnér 9. Metabolic labeling with radioactive selenium in zebrafish Paul Copeland 10. Low pH isoTOP-ABPP to identify selenocysteines Daniel Bak 11. Expression of selenoproteins via genetic code expansion in mammalian cells Jennifer Peeler 12. Alpha-methyl selenocysteine as a tool for the study of selenoproteins Robert Hondal 13. Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications Jianguo Fang and Baoxin Zhang 14. Diselenide-selenoester ligation Richard Payne 15. Modeling selenocysteine-derived reactive intermediates utilizing a molecular cradle Kei Goto 16. Methods for accurate and reproducible studies of pharmacological effects of selenium in cancer Mikael Björnstedt