Estrogen Receptors

Antibody Validation for Estrogen Receptor Beta.- Immunoblot Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30/GPER1 Activation.- Immunoprecipitation Analyses of Estrogen Receptor α Phosphorylated at Serine 216 in the Mouse Liver.- Immunofluorescent Verification of Silencing Estrogen Receptor α with siRNA in the Intact Rodent Brain.- Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils.- Quantification of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate in Response to Estrogen.- In Situ Hybridization of Estrogen Receptors α and β in the Human Testis.- Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR.- PET Imaging of Estrogen Receptors Using 18F-Based Radioligands.- The Use of ERE-Luc Reporter Mice to Monitor Estrogen Receptor Transcriptional Activity in a Spatio-Temporal Dimension.- Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model.- Assessing Estrogenic Activity of Classical Estrogen Receptor‑Binding Compounds.- An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta.- Polysome-Fractionation for Transcriptome-Wide Studies of mRNA Translation.- Anota2seq Analysis for Transcriptome-Wide Studies of mRNA Translation.- Whole-Genome Genotyping Using DNA Microarrays for Population Genetics.- Proteomics Analysis of the Estrogen Effects in the Rat Uterus Using Gel-LC and Tandem Mass Spectrometry Approaches.- Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells.- Molecular Cloning and Purification of the Protein Lysine Methyltransferase SMYD2 and Its Co-Crystallization with a Target Peptide from Estrogen Receptor Alpha.- Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Targeted Protein Degradation of ERα.- Assessment of Metabolic Regulation by Estrogen Receptors.- RNA-Seq Experiment and Data Analysis.