Planar Gel Electrophoresis

Dr Teck Yew Low is currently an Associate Professor and Senior Research Fellow at the UKM Medical Molecular Biology Institute (UMBI), National University of Malaysia (UKM), where he leads the cancer proteomics and metabolomics group. He obtained his PhD in Biochemistry from the National University of Singapore (NUS) and worked with mass spectrometry-based proteomics in the Genome Institute of Singapore (GIS), the Center for Experimental Bioinformatic (CEBI) in University of Southern Denmark (SDU) and the Netherlands Proteomics Center (NPC) in Utrecht University in the Netherlands. His research interests include bridging proteomics with clinics, phosphoproteomics and cancer proteogenomics in the Malaysian population.

Section 1: History, Theory and Milestones
1. Historical backgrounds and milestones
2. Theory and core concepts
3. Different gel chemistry and buffer systems

Section 2: Sample preparation
4. Preparation of protein samples

Section 3: Different electrophoretic techniques
5. SDS-PAGE (Denaturing gels)
6. Non-denaturing gels
7. Non-reducing gels
8. Gradient gels to cover high MW and low MW proteins
9. Separation of low MW peptides with Tris-Tricine gels
10. Agarose Gels for Separating High MW Proteins
11. Preparative gels
12. Acid- urea – triton gel for separating baisc proteins and histones
13. Blue native gels for separating protein complex
14. Pore gradient gel electrophoresis (PGGE)
15. Cationic Electrophoresis
16. Isoelectric focusing (IEF)
17. Immobilized pH gradients (IPG)
18. Two-dimenstional gel electrophoresis (2-DE)
19. Difference gel electrophoresis (DIGE)
20. Diagonal Electrophoresis for disulfide bond detection
21. Affinity gel electrophoresis (AEP)
22. Electrophoretic mobility shift assays (EMSA)
23. Immobilized metal affinity electrophoresis (IMAEP)
24. 1D and 2D zymography (1DZ and 2DZ)
25. SARCOSYL-PAGE: separation of PEGylated proteins
26. Phenylboronate acrylamide gel electrophoresis for glycated proteins
27. Field Inversion gel electrophoresis (FIGE)
28. Microfluidic gel electrophoresis

Section 4: Post-electrophoresis analysis
29. Visualization of gel separated proteins with protein stains
30. Densitometry analysis of protein bands or spots
31. Data analysis software for extracting information from gel separations
32. Recovery of proteins from gel with electroelution
33. In-gel proteolytic digestion of proteins and mass spectrometry

Section 5: Coulping electrophoresis with antibody detection
34. Western Blotting
35. Serological Proteome Analysis (SERPA)
36. Immunofixation Electrophoresis (IFE)
37. Immunoelectrophoresis (IEP)