Fusarium wilt

1.  Pure culture and DNA sequence-based identification of Fusarium from symptomatic plants and diverse substrates.- Isolation of high-molecular weight (HMW) DNA from Fusarium oxysporum for long-read sequencing.- Karyotyping of Fusarium oxysporum by pulsed field gel electrophoresis and the germ tube burst method.- RNA-seq data analysis for differential expression.- Quantification and isolation of spontaneous colony growth variants.- Generation of a T-DNA insertion mutant library of Fusarium oxysporum using Agrobacterium tumefaciens.- Targeted gene disruption via CRISPR/Cas9 ribonucleoprotein complexes in Fusarium oxysporum.- CRISPR/Cas9 RNP-mediated gene fusion to assess protein quantification and subcellular localization in Fusarium oxysporum.- In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin.- Multi-pronged investigation of volatile compound-mediated interactions of Fusarium oxysporum with plants, fungi and bacteria.- Isolation of mitochondria from Fusarium species.- In planta gene expression analysis and colonization of Fusarium oxysporum.- Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots.- High-throughput screening assays to identify plant natural products with antifungal properties against Fusarium oxysporum.- Screening and assessment of pisatin demethylase activity (PDA).-Detection and genotyping of Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), the Fusarium wilt pathogen of cotton.