Group A Streptococcus
Springer-Verlag New York Inc.
978-1-0716-0469-4 (ISBN)
Authoritative and cutting-edge, Group A Streptococcus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Detection of Streptococcus pyogenes Virulence Factors.- Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens.- The emm-cluster Typing System.- Protocols for Tn-seq Analyses in The Group A Streptococcus.- Genetic Manipulation of Group A Streptococcus – Gene Deletion by Allelic Replacement.- Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging.- Whole Genome Sequence Analysis and Population Genomics of Group A streptococci.- Group A Streptococcus Transcriptome Analysis.- Protocol for Proteome Analysis of Group A Streptococcus.- Investigation of Group A Streptococcal Interactions with Host Glycan Structures using High Throughput Techniques: Glycan Microarray Analysis using Recombinant Protein and Whole Cells.- Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins.- Expression and Purification of Collagen-like Proteins of Group A Streptococcus.- Detection of Fibronectin-binding Proteins of Streptococcus pyogenes using Ligand Blot Analysis.- Morphology and Ultrastructure of Group A Streptococcus Biofilms.- Dynamic Interactions of Group A Streptococcus with Host Macrophages.- Identification of Group A Streptococcus-containing autophagosome-like vacuoles.- Flow Cytometry-based Assays to Quantify Complement Deposition and Neutrophil Uptake of Group a Streptococcus.- Live Cell Microscopy and Flow Cytometry to study Streptolysin S Mediated Erythrocyte Hemolysis.- Isolation of Monoclonal Antibodies to Group A Streptococcus Antigens Using Phage Display.- Assays to Analyse Adhesion of Group A Streptococcus to Host Cells.- The use of Galleria mellonella (wax moth) as an Infection Model for Group A Streptococcus.- A Superficial Skin Scarification Method in Mice to Mimic Streptococcus pyogenes Skin Infection in Humans.- A Mouse Nasopharyngeal Colonisation Model for Group A Streptococcus.- Humanised Plasminogen Mouse Model to Study Group A Streptococcus Invasive Disease.- Lacefield Whole Blood Killing Assay to Evaluate Vaccine Efficacy.- An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera.- Validation of Suitable Carrier Molecules and Target Genes for antisense Therapy using Peptide-coupled Peptide Nucleic Acids (PNAs) in Streptococci.- Interfering with the Folding of Group A Streptococcal pili Proteins.- Use of Streptolysin O (SLO) to Study the Function of Lipid Rafts.- Engineering of Group A Streptococcus Isopeptide Bonds into Immunoglobulin-like Protein Domains.
Erscheinungsdatum | 28.06.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2136 |
Zusatzinfo | 39 Illustrations, color; 15 Illustrations, black and white; XIV, 403 p. 54 illus., 39 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-0716-0469-4 / 1071604694 |
ISBN-13 | 978-1-0716-0469-4 / 9781071604694 |
Zustand | Neuware |
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