Molecular Toxicology Protocols

Bioenergetic Analyses of In Vitro and In Vivo Samples to Guide Toxicological Endpoints.- Next Generation Sequencing (NGS) Application in Multi-Parameter Gene Expression Analysis.- Integrated Chip-Seq and RNA-Seq Data Analysis Coupled with Bioinformatics Approaches to Investigate Regulatory Landscape of Transcription Modulators in Breast Cancer Cells.- Analysis of RNA Sequencing Data using CLC Genomics Workbench.- Using Bronchoalveolar Lavage to Evaluate Changes in Pulmonary Diseases.- Fast and Efficient Measurement of Clinical and Biological Samples Using Immunoassay-Based Multiplexing Systems.- Induction of Lung Tumors and Mutational Analysis in FVB/N Mice Treated with the Tobacco Carcinogen 4-(Methylnitrosamino)-1-(3-pyridyl)-1-Butanone.- Two-Dimensional Difference Gel Electrophoresis: A Gel-Based Proteomic Approach for Protein Analysis.- Determination and Quantification of Bacterial Virulent Gene Expression Using Quantitative Real Time PCR.- Using a Lentivirus-Based Inducible RNAi Vector to Silence a Gene.- Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).- Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques.- Analysis of Telomere Length and Aberrations by Quantitative FISH.- Detection of Loss of Heterozygosity in Tk-Deficient Mutants from L5178Y Tk+/–-3.7.2C Mouse Lymphoma Cells.- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR.- 32P-Postlabeling Analysis of DNA Adducts.- Determination of Mutational Spectra Induced by Environmental Toxicants in Complex Human Cell Populations.- Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice.- Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes.- Molecular Analysis of Mutations in the Human HPRT Gene.- Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions In Human Lymphocytes.- Analysis of Mutations in K-ras and p53 Genes in Sputum and Plasma Samples.- ACB-PCR Quantification of Low-Frequency Hotspot Cancer-Driver Mutations.- Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions.- The Sister Chromatid Exchange (SCE) Assay.- The Gene Cluster Instability (GCI) Assay for Recombination.- New Perspectives on Unscheduled DNA Synthesis:  Functional Assay for Global Genomic DNA Nucleotide Excision Repair.- Host Cell Reactivation: Assay for Actively Transcribed DNA Nucleotide Excision Repair Using Luciferase Family Expression Vectors.- Methods for Establishing and Using a Stable Cell Line Expressing Both Gaussia Luciferase and Firefly Luciferase to Screen for Endoplasmic Reticulum Stress.- FREMSA: A Method That Provides Direct Evidence of the Interaction Between MicroRNA and mRNA.