Ion Channels: Channel Biochemistry, Reconstitution, and Function

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Preface

Daniel L. Minor Jr. and Henry M. Colecraft

Structure/function relationships

1. Correlating ion channel structure and function

Crina Nimigean

2. TRPV3 Expression and Purification for Structure Determination by Cryo-EM

Alexander Sobolevsky

3. Methods to study phosphoinositide regulation of ion channels

Tibor Rohacs

4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies

Sudha Chakarpani

5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography

Anna Moroni

Structural and chemical methods to assess channel dynamics

6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy

Simon Scheuring

7. Crosslinking for gating motions in GluRs

Andrew Plested

8. Single molecule FRET methodology for investigating glutamate receptors

Vasanthi Jayaraman

9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues

Murali Prakriya

Channel reconstitution and studies in bilayers

10. A quantitative flux assay for the study of Cl- channels and transporters

Alessio Accardi

11. Ion channels reconstitution in lipid bilayers

Eleonora Zakharian

12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers

Gehard Thiel

Methods to assess oligomerization

13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture

Randy Stockbridge