Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Preface

Daniel L. Minor Jr. and Henry M. Colecraft

Methods to engineer ion channels to probe different aspects of channel structure-function and regulation.

1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern

2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless

3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung

4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura

5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx

Methods to control and characterize ion channel function

6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft

7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny

8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein

9: Stretch and poke stimulation for characterizing mechanically activated ion channels. Jörg Grandl

10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU

11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS

Methods to characterize ion channel function and expression in physiology and disease

12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko

13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea

14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge

15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang

16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr.

17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy

Nenad Bursac