Mass Spectrometry of Glycoproteins

Glycosylation of Therapeutic Proteins: A Critical Quality Attribute.- Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass Spectrometry.- Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.- Fc Glycosylation Characterization of Human Immunoglobulins G using Immunocapture and LC-MS.- Fast Afucosylation Profiling of Glyco-Engineered Antibody Subunits by Middle Up Mass Spectrometry.- Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS.- Analysis of Monoclonal Antibody Glycopeptides by Capillary Electrophoresis – Mass Spectrometry Coupling (CE-MS).- Enrichment of Intact Glycopeptides using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography.- In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC –SPE and LC-MS/MS.- Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MSE.- Mapping O-glycosylation Sites using OpeRATOR and LC-MS.- Site-Specific O-Glycosylation Analysis by Liquid Chromatography/Mass Spectrometry with Electron-Transfer/Higher-Energy Collisional Dissociation.- Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS after Derivatization with Fluorescent Anthranilamide.- Evaluating N-glycosylation of a Therapeutic Monoclonal Antibody using UHPLC-FLR-MS with RapiFluor-MS Labeling.- Routine Analysis of N-glycans using Liquid Chromatography Coupled to Routine Mass Detection.- Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals.- Profiling, Relative Quantification, and Identification of Sialylated N-Linked Oligosaccharides by UPLC-FLR-ESI/MS after Derivatization with Fluorescent Anthranilamide.- Linkage Analysis of Oligo- and Polysaccharides: A Tutorial.- Use of Exoglycosidases for the Structural Characterization of Glycans.- Determination of Isomeric Glycan Structures by Permethylation and Liquid Chromatography-Mass Spectrometry (LC-MS).- Optimization of Multiple Glycosidase and Chemical Stabilization Strategies for N-glycan Isomer Detection by Mass Spectrometry Imaging in Formalin-Fixed, Paraffin-Embedded Tissues.- Glycosylation Profiling of Glycoproteins Secreted from Cultured Cells Using Glycan Node Analysis and GC-MS.- Array-Based N-Glycan Profiling of Cells in Culture.- Analysis of Oligomeric and Glycosylated Proteins by Size-Exclusion Chromatography Coupled with Multi-Angle Light Scattering.- Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.- Deploying Mass Spectrometric Data Analysis in the Amazon AWS Cloud Computing Environment.