Gene Biotechnology, Second Edition - William Wu, Helen H. Zhang, Michael J. Welsh, Peter B. Kaufman

Gene Biotechnology, Second Edition

Buch | Hardcover
536 Seiten
2003 | 2nd New edition
Crc Press Inc (Verlag)
978-0-8493-1288-5 (ISBN)
119,95 inkl. MwSt
zur Neuauflage
  • Titel erscheint in neuer Auflage
  • Artikel merken
Zu diesem Artikel existiert eine Nachauflage
Offers a multidisciplinary approach to the methods essential to biotechnical development. Presenting information that researchers and students need to understand and apply biotechnology techniques, this work discusses a variety of approaches, from basic methods to the sophisticated technologies, including gene manipulation methods.
Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development.

Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects.

Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.

Approaches to Novel Research.
Rapid Isolation of Specific cDNAs or Genes by PCR
New! High Throughput mRNA Profiling via cDNA Microarrays or DNA Chips
New! mRNA Profiling by Quantitative RT-PCR Technique
New! Functional Genome vs. Drug Discovery
Construction and Screening of Subtracted and Complete Expression cDNA Libraries
Subcloning of Gene or cDNAs
Nonisotopic and Isotopic DNA or RNA Sequencing
Information Superhighway and Computer Databases of Nucleic Acids and Proteins
Characterization of DNA or Genes by Southern Blot Hybridization
Gene Overexpression by Sense RNA in Mammalian Systems
Analysis of Gene Expression at the RNA Level
Analysis of Gene Expression at the Protein Level
New! Proteomics vs. Drug Discovery
New! Phage Display Technology
DNA or mRNA Analysis by Radioactive in situ Hybridization RT-PCR Detection of Low Abundance mRNAs
Isolation and Characterization of Genes from Genomic DNA Libraries
Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression Studies
New Strategies for Gene Knockout
Generation of Random Chimeras for Functional Assays of Genes
Rapid Generation of Mutant DNA Constructs by Site-Specific Mutagenesis
Large-scale Expression and Purification of Recombinant Proteins

Erscheint lt. Verlag 29.9.2003
Zusatzinfo 57 Tables, black and white; 124 Illustrations, black and white
Verlagsort Bosa Roca
Sprache englisch
Maße 156 x 235 mm
Gewicht 871 g
Themenwelt Naturwissenschaften Biologie Genetik / Molekularbiologie
Technik Umwelttechnik / Biotechnologie
ISBN-10 0-8493-1288-4 / 0849312884
ISBN-13 978-0-8493-1288-5 / 9780849312885
Zustand Neuware
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich