Dark Art of Blood Cultures (eBook)

Wm. Michael Dunne, Jr., PhD, is currently a senior research fellow for bioMérieux, Inc. in Durham, North Carolina. Priorto joining bioMérieux, he was Medical Director of the Diagnostic Microbiology Laboratory at Barnes-Jewish Hospital and Professor of Pathology and Immunology, Molecular Microbiology, Pediatrics, and Medicine at Washington University School of Medicine in St. Louis from 2000-2011 and remainson the faculty there. During that time, he established an internationally-recognized training program in medical and public health microbiology, which is still active under the direction of Carey-Ann Burnham. He has previously served as Medical Director of Microbiology Laboratories at Henry Ford Hospital (Detroit, Michigan), Texas Children's Hospital (Baylor College of Medicine, Houston, Texas) and Children'sHospital of Wisconsin (Medical College of Wisconsin, Milwaukee, Wisconsin, where he had received his PhD in 1982). Dr. Dunne is a Diplomate of the American Board of Medical Microbiology and a Fellow of the American Academy of Microbiology, the Infectious Diseases Society of America, the Pediatric Infectious Diseases Society, and the Canadian College of Microbiology. He served as a senioreditor of the Journal of Clinical Microbiology for ten years and remains on the editorial board. He is also an Adjunct Professor of Pediatrics at Duke University School of Medicine, Durham, North Carolina. He has authored or coauthored over 170 peer-reviewed publications and 10 chapters, in addition to this book. Carey-Ann D. Burnham, PhD, completed her PhD in Medical Sciences at the University of Alberta in Edmonton, Alberta, Canada and her postdoctoral training in Medical and Public Health Microbiology at Washington University under the direction of Wm. Michael Dunne, Jr. Currently, Burnhamis an Associate Professor of Pathology & Immunology, Molecular Microbiology, and Pediatrics at Washington University in St. Louis School of Medicine. Burnham has a keen interestin education and is the Program Director for the Medical and Public Health Microbiology Fellowship at Washington University, the co-editor of Medical Microbiology Question of the Day, and the section editor of The Brief Case for the Journal of Clinical Microbiology. Burnham's research program focuses on development of diagnostic assays for infectious diseases and the transmission and epidemiology of antimicrobial resistance in bacteria. Burnham has authored or coauthored more than 100 peer-reviewed publications in addition to numerous invited articles and book chapters.

Contributors

Foreword

Preface

Acknowledgments

About the Editors

I Perspectives on the Art and Science of Blood Culture

1 Historical Perspectives on the Art and Science of Blood Culture
William Michael Dunne, Jr.

2 Conventional Blood Culture Methods
Robyn Atkinson-Dunn and William Michael Dunne, Jr.

3 Lysis-Centrifugation Methods of Blood Culture
Erin McElvania TeKippe and Morgan A. Pence

4 BACTEC Blood Culture Systems
Robin R. Chamberland

II Modern Methods in Blood Culture

5 The BioMérieux BacT/ALERT: Automation at Last in the Black Box
Bradley Ford and George Kallstrom

6 TREK Blood Culture Systems
Neil W. Anderson and Melanie Yarbrough

7 Molecular Methods for Detection of Pathogens Directly from Blood Specimens
Mark D. Gonzalez and Robert C. Jerris

8 Pediatric Blood Cultures
Paula Revell and Christopher Doern

9 Epidemiology of Bloodstream Infections
Allison R. McMullen, Craig B. Wilen, and Carey-Ann D. Burnham

10 Best Practices in Blood Cultures
Robert J. Tibbetts and Barbara Robinson-Dunn

11 Processing Positive Cultures
Matthew L. Faron and Nathan A. Ledeboer

12 Fungal and Mycobacterial Blood Cultures
Robert S. Liao and William Lainhart

IV New Developments in Blood Culture

13 The Bacterial Blood Microbiota/Microbiome
Eileen M. Burd and Lars F. Westblade

Index