Neural Crest Cells

The neural crest: a remarkable model system for studying development and disease.- Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example.- Generation of neural crest progenitors from human pluripotent stem cells.- Fully defined and xeno-free induction of hPSCs into neural crest using top-down inhibition of BMP signaling.- Deciphering the neural crest contribution to cephalic development with avian embryos.- In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis.- Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants.- Technologies for live imaging of enteric neural crest derived cells.- Mouse embryo culture for the study of neural crest cells.- Laser capture microdissection of murine embryonic neural crest cells.- In vivo and in vitro quantitative analysis of neural crest cell migration.- Proteomics in neural crest cell research.- Neural crest methodologies in zebrafish and medaka.- Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells.- Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity.- Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective.