DNA Repair

Homologous Recombination-Mediated DNA Repair and Implications for Clinical Treatment of Repair Defective Cancers.- Resolving Roadblocks to Telomere Replication.- In Time and Space: Laser Micro-Irradiation and the DNA Damage Response.- Quantification of Double Strand Breaks in Mammalian Cells using Pulsed-Field Electrophoresis.- Methods to Study Trinucleotide Repeat Instability Induced by DNA Damage and Repair.- Extracting and Measuring dNTP Pools in Saccharomyces cerevisiae.- Conversion Tract Analysis of Homology-Directed Genome Editing using Oligonucleotide Donors.- Reporter Assays for BER Pathways.- Methods for Studying DNA Single-Strand Break Repair and Signaling in Xenopus laevis Egg Extracts.- Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts.- Cellular Assays to Study the Functional Importance of Human DNA Repair Helicases.- A Mammalian Genetic Complementation Assay for Assessing Cellular Resistance to Genotoxic Compounds.- Small Molecule Inhibitor Screen for DNA Repair Proteins.- Assembling the Human Resectosome on DNA Curtains.- Thin-Layer Chromatography and Real-Time Coupled Assays to Measure ATP Hydrolysis.- Gel-Based Assays for Measuring DNA Unwinding, Annealing, and Strand Exchange.- In Vitro Assay for Plasmid Length DNA Strand Exchange by Human DMC1.- In Vitro Assays for DNA Branch Migration.- Stabilization of Human DMC1 Nucleoprotein Filament.- Measuring UV Photoproduct Repair in Isolated Telomeres and Bulk Genomic DNA.- Single-Molecule DNA Fiber Analyses to Characterize Replication Fork Dynamics in Living Cells.- Direct Visualization of DNA Replication at Telomeres using DNA Fiber Combing Combined with Telomere FISH.- Telomere and G-Quadruplex Co-Localization Analysis by Immunofluorescence-Fluorescence In Situ Hybridization (IF-FISH).- FISHing for Damage on Metaphase Chromosomes.