Cell-Based Assays Using iPSCs for Drug Development and Testing
Humana Press Inc. (Verlag)
978-1-4939-9479-3 (ISBN)
Cell-Based Assays using Differentiated Human Induced Pluripotent Cells.- Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells.- Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modelling.- Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation.- Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies.- Improved Protocol of Cardiac Differentiation and Maturation of Pluripotent Stem Cells.- Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors.- A Protocol for the One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes.- Differentiation of hiPS Cells into Definitive Endoderm for High Throughput Screening.- LC-MS/MS Based Metabolomics for Cell Cultures.- Preparation of iPSCs for Targeted Proteomic Analysis.- Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes.- Mass Spectrometry Measurement of Albumin/Alpha Fetoprotein Ratio as an Indicator of iPSC-Derived Hepatocyte Differentiation.- Seeding Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well Plates.- High Content Autophagy Analysis in iPSC-Derived Neurons using Immuno-Fluorescence.- High Content Analysis of Mitochondrial Function in iPSC-Derived Neurons.- Evaluating the Effect of Drug Compounds on Cardiac Spheroids using the Cardiac Cell Outgrowth Assay.- Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes using Multi-Well Microelectrode Arrays.- Multielectrode Array (MEA) Based Detection of Spontaneous Network Activity in Human iPSC-Derived Cortical Neurons.- Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm.- Fabrication of a Microfluidic Cell Culture Device using Photolithographic and Soft Lithographic Techniques.- Using a Microfluidic Device for Culture and Drug Toxicity Testing of 3D Cells.- hiPS Cell–Derived Neurons for High-Throughput Screening.
Erscheinungsdatum | 07.07.2020 |
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Reihe/Serie | Methods in Molecular Biology ; 1994 |
Zusatzinfo | 55 Illustrations, color; 14 Illustrations, black and white; XIV, 266 p. 69 illus., 55 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie |
Medizin / Pharmazie ► Pharmazie | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | Cardiac cells • Hepatocytes • Human induced pluripotent cells • Kidney cells • neuronal cells • pharmaceuticals |
ISBN-10 | 1-4939-9479-4 / 1493994794 |
ISBN-13 | 978-1-4939-9479-3 / 9781493994793 |
Zustand | Neuware |
Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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