Microbial Transposon Mutagenesis

Protocols and Applications

Steven C. Ricke, Si Hong Park, Morgan L. Davis (Herausgeber)

Buch | Softcover

183 Seiten

2020 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-9572-1 (ISBN)

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This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.

Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium.- Application of Whole Genome Sequencing to Transposon Mutants of Salmonella Heidelberg.- Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus.- Transposon Mutagenesis in Streptococcus Species.- Implementation of Transposon Mutagenesis in Bifidobacterium.- Transposon Mutagenesis of Listeria monocytogenes.- Transposon Mutagenesis of Foodborne Pathogenic Esherichia coli.- Methods for Transposon Mutagenesis in Proteus mirabilis.- Mutagenesis of Vibrio fischeri and Other Marine Bacteria Using Hyperactive Mini-Tn5 Derivatives.- Transposon Mutagenesis of Bacteroides fragilis.- Transposon Mutagenesis in Mycobacterium avium Subspecies Paratuberculosis.- Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches.- Construction of DNA Barcode-Tagged Salmonella Strains.- Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples.- Efficient Gene Deletion Method for Listeria monocytogenes.- Whole Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools.

Erscheinungsdatum	07.07.2020
Reihe/Serie	Methods in Molecular Biology ; 2016
Zusatzinfo	34 Illustrations, color; 12 Illustrations, black and white; XI, 183 p. 46 illus., 34 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Bifidobacterium • Bioinformatic tools • Microorganism genetics • Phenotypic characteristics • Salmonella • Transposon mutant libraries • whole genome sequencing
ISBN-10	1-4939-9572-3 / 1493995723
ISBN-13	978-1-4939-9572-1 / 9781493995721
Zustand	Neuware