Light Microscopy in Biology

Since the first edition of "Light Microscopy in Biology: A Practical Approach" was published, techniques in modern light microscopy have improved considerably. This edition includes revised topics from the first book along with additional material including chromosome microscopy and image building.

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Since the first edition of "Light Microscopy in Biology: A Practical Approach" was published, techniques in modern light microscopy have improved considerably. This updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that have been developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy; immunohistochemistry; fluorescence microscopy; image building; and video microscopy. Additional topics covered include: confocal microscopy; calcium and pH imaging; microinjection techniques; and nanovid microscopy. There are also chapters devoted to reflection contrast microscopy and histomorphometry. The volume should be of interest to postgraduate and postdoctoral researchers in biomedicine and cell biology - both those experienced with light microscopic techniques and newcomers to the field.