Molecular and Cellular Methods in Developmental Toxicology - George P. Daston

Molecular and Cellular Methods in Developmental Toxicology

Buch | Hardcover
304 Seiten
2020
CRC Press (Verlag)
978-1-138-41723-6 (ISBN)
143,40 inkl. MwSt
A manual of experimental procedures, including frequently-used molecular and cellular techniques. Experimental protocols have been selected by developmental toxicologists. Diagrams illustrate all procedures, and there are photographs of experimental results.
This new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research.

Daston, George P.

Part I. Molecular Techniques -- Chapter 1. Molecular Techniques for Developmental Toxicology: An Introduction/George P Daston -- Chapter 2. Visualization of Gene Expression Patterns by In Situ Hybridization/ Urs Albrecht, Gregor Eichele, Jill A. Helms, and Hui-Chen Lu -- Chapter 3. Utilizing Transgenic Mice to Study Gene Regulation and Function/Michael R. Blackburn and Rodney E. Kellems -- Chapter 4. The Use of Antisense Oligonucleotide Technology and Mouse Whole-Embryo Culture to Study Gene Function During Organogenesis/Linda Foerst Potts and T W. Sadler -- Chapter 5. Nucleic Acid Amplification Technologies/Richard H. Finnell, Scott J. Vacha, and Scott A. Mackler -- Part II. Protein Identification -- Chapter 6. Fundamentals of Protein Identification: Biochemical and Immunological Analysis of Developmentally Altered Protein Expression/Gerald B. Grunwald -- Chapter 7. Immunohistochemistry/Philip E. Mirkes and Sally A Little -- Part III. Cellular Techniques -- Chapter 8. Cellular Techniques for Teratological Cell Death/Thomas B. Knudsen -- Chapter 9. Applications of Flow Cytometry in Developmental and Reproductive Toxicology/Kenneth H. Elstein and Robert M. Zucker -- Chapter 10. Modern Techniques for Cell Labeling in Avian and Murine Embryos/Diana K. Darnell and Gary C. Schoenwolf -- Index.

Erscheinungsdatum
Reihe/Serie Methods in Life Sciences - Toxicology Section
Verlagsort London
Sprache englisch
Maße 156 x 234 mm
Gewicht 720 g
Themenwelt Studium 2. Studienabschnitt (Klinik) Pharmakologie / Toxikologie
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Genetik / Molekularbiologie
ISBN-10 1-138-41723-8 / 1138417238
ISBN-13 978-1-138-41723-6 / 9781138417236
Zustand Neuware
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