Extracellular Vesicles

Sheila Spada is at Weill Cornell Medical College, NY, USA Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. Genetic labeling of extracellular exosomes for studying biogenesis and uptake in living mammalian cells

Daniel Levy, Mai Anh Do, Annie Brown, Kyle Asano, David Diebold, Hanzhe Chen, Jiayi Zhang, Brendan Lawler and Biao Lu

2. Fluorescent labeling of extracellular vesicles

Mehdi Dehghani and Thomas R. Gaborski

3. Use of antibody arrays to probe exosome and extracellular vesicle mediated functional changes in cells

Grazia Ambrosini, Blake Ebert, Richard D. Carvajal, Gary K. Schwartz and Alex J. Rai

4. Analysis of individual extracellular vesicles by imaging flow cytometry

Tobias Tertel, André Görgens and Bernd Giebel

5. Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light sheet fluorescence microscopy

Sara Elgamal, Federico Colombo, Francesca Cottini, John C. Byrd and Emanuele Cocucci

6. Methods to purify DNA from extracellular vesicles: Focus on exosomes

Sheila Spada

7. Quantification of mtDNA in extracellular vesicles

Beata Soltes and Balint Nagy

8. Deciphering the total RNA content of extracellular vesicles

Maria G. Amorim, Emmanuel Dias-Neto and Diana N. Nunes

9. Immunocapture-based ELISA to characterize and quantify exosomes in both cell culture supernatants and body fluids

Mariantonia Logozzi, Rossella Di Raimo, Davide Mizzoni and Stefano Fais

10. Isolation and characterization of human amniotic fluid-derived exosomes

Samantha Sheller-Miller and Ramkumar Menon

11. Human urine exosomes: Another important member of the liquid biopsy family

Antonio De Maio

12. Three-dimensional cell models for EV production, isolation, and characterization

Liliia Paniushkina, Elena Grueso Navarro, Xu Chen and Irina Nazarenko

13. Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles

Aya Yoshimura, Yoshitaka Tamai and Takahiro Ochiya

14. Live tracking of extracellular vesicles in larval zebrafish

Benjamin Mary, Shima Ghoroghi, Vincent Hyenne and Jacky G. Goetz

15. Assessment of innate immune response activation following the injection of extracellular vesicles isolated from human cell cultures in zebrafish embryos

Marco Cafora, Mirjam Hoxha, Laura Cantone, Valentina Bollati, Anna Pistocchi and Luca Ferrari