Channelrhodopsin

Methods and Protocols

Buch | Hardcover

390 Seiten

2020
Springer-Verlag New York Inc.
978-1-0716-0829-6 (ISBN)

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This book merges approaches in understanding the function of the light-gated ion channels known as channelrhodopsin together with methods addressing how channelrhodopsins can be used to address biomedical questions on a cellular or organismal level. Since the first molecular identification of channelrhodopsins, a broad range of tools have been created and new approaches developed to both better understand the molecular determinants of channelrhodopsin function as well as to use these and homologous proteins from a variety of species as tools to better understand physiological processes, which this volume addresses. Additionally, channelrhodopsins have become instrumental as a potential treatment for disease states. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Channelrhodopsin: Methods and Protocols provides a resource for those interested in honing their current expertise in this vital area of study as well as potentially branching out into new directions.

Part I: Basic Science

1. Molecular Dynamics Simulations of Channelrhodopsin Chimera, C1C2
Monika R. VanGordon

2. Computing Potential of the Mean Force Profiles for Ion Permeation through Channelrhodopsin Chimera, C1C2
Chad Priest, Monika R. VanGordon, Caroline Rempe, Mangesh Chaudhari, Mark Stevens, Steve Rick, and Susan B. Rempe

3. Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in Pichia pastoris for Biophysical Investigations
Maria Walter and Ramona Schlesinger

4. Characterizing Channelrhodopsin Channel Properties via Two-Electrode Voltage Clamp and Kinetic Modeling
Lindsey Prignano, Lauren Herchenroder, and Robert E. Dempski

Part II: Cells

5. Charge Transport by Light-Activated Rhodopsins Determined by Electrophysiological Recordings
Tamara Hussein and Christian Bamann

6. Probing Channelrhodopsin Electrical Activity in Algal Cell Populations
Oleg A. Sineshchekov, Elena G. Govorunova, and John L. Spudich

7. Two-Photon Optogenetic Stimulation of Drosophila Neurons
Mehmet Fisek and James M. Jeanne

8. Probing Synaptic Signaling with Optogenetic Stimulation and Genetically-Encoded Calcium Reporters
Gabriel B. Borja, Himali Shroff, Hansini Upadhyay, Pin W. Liu, Valeriya Baru, Yung-Chih Cheng, Owen B. McManus, Luis A. Williams, Graham T. Dempsey, and Christopher A. Werley

9. Optogenetics to Interrogate Neuron-Glia Interactions in Pups and Adults
Chloe Habermacher, Blandine Manot-Saillet, Domiziana Ortolani, Fernando C. Ortiz, and Maria Cecilia Angulo

10. Chronic Optogenetic Pacing of Human Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues
Marc Dwenger, William J. Kowalski, Hidetoshi Masumoto, Takeichiro Nakane, and Bradley B. Keller

Part III: Animals

11. Patterned Optogenetic Stimulation Using a DMD-Projector
Aanchal Bhatia, Sahil Moza, and Upinder S. Bhalla

12. Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in Differing Light Conditions
Tushar H. Ganjawala and Zhuo-Hua Pan

13. Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish
Yagmur Idil Ozdemir, Christina A. Hansen, Mohamed A. Ramy, Eileen L. Troconis, Lauren D. McNeil, and Josef G. Trapani

14. Automated Functional Screening for Modulators of Optogenetically-Activated Neural Responses in Living Organisms
Ross C. Lagoy, Eric Larsen, and Dirk R. Albrecht

15. Optogenetic Interrogation of ChR2-Expressing GABAergic Interneurons after Transplantation into the Mouse Brain
Muhammad N. Arshad, Gloster B. Aaron, and Janice R. Naegele

16. Application of Targeting-Optimized Chronos for Stimulation of the Auditory Pathway
Antoine Huet and Vladan Rankovic

17. Channelrhodopsins for Cell-Type Specific Illumination of Cardiac Electrophysiology
Marbely C. Fernandez, Ramona A. Kopton, Ana Simon Chica, Josef Madl, Ingo Hilgendorf, Callum M. Zgierski-Johnston, and Franziska Schneider-Warme

18. Optogenetic Control of Cardiac Autonomic Neurons in Transgenic Mice
Angel Moreno, Grant Kowalik, David Mendelowitz, and Matthew W. Kay

19. Dissecting Mechanisms of Motivation within the Nucleus Accumbens Using Optogenetics
Shannon L. Cole and Jeffrey J. Olney

20. Optogenetic Stimulation of the Central Amygdala Using Channelrhodopsin
Anna S. Knes, Charlotte M. Freeland, and Mike J.F. Robinson

21. Optical Manipulation of Perfused Mouse Heart Expressing Channelrhodopsin-2 in Rhythm Control
Xi Wang and Yue Cheng

22. Chronic Optogenetic Stimulation in Freely-Moving Rodents
Thiago C. Moulin

Erscheinungsdatum	04.09.2020
Reihe/Serie	Methods in Molecular Biology ; 2191
Zusatzinfo	109 Tables, color; 109 Illustrations, color; 7 Illustrations, black and white
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Gewicht	983 g
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
ISBN-10	1-0716-0829-0 / 1071608290
ISBN-13	978-1-0716-0829-6 / 9781071608296
Zustand	Neuware