Leukemia Stem Cells

Introduction and Classification of Leukemias.- Leukemia Stem Cells: Concept and Implications.- Leukemia Stem Cell Drug Discovery.- Analysis and Isolation of Mouse Leukemic Stem Cells.- Mass Cytometry of Hematopoietic Cells.- PCR Technology to Identify Minimal Residual Disease.- Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies.- Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology).- Single-Cell Transcriptomic Analysis of Hematopoietic Cells.- In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics.- In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation.- Experimental Competitive Bone Marrow Transplant Assays.- Human T-ALL Xenografts.- An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts.- Leukemic Stem Cell Culture in Cytokine-Free Medium.- Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid.- Isolation, Culture, and Manipulation of Human Cord Blood Progenitors.- Lentiviral Transduction for Optimal LSC/HSC Manipulation.- Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes.- Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes.- Arrayed Molecular Barcoding of Leukemic Stem Cells.- In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis.- In Situ Hematopoietic Stem Cell Imaging.- A Genome-Editing System for Therapeutical Targeting of Stem Cells.- Method for the Generation of Induced Hematopoietic Stem Cells.- Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells.- High Content Imaging to Phenotype Human Primary and iPSC-Derived Cells.- Bioinformatic Methods to Identify Mutational Signatures in Cancer.