Immunometabolism

Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq.- Monitoring Lactate Dynamics in Individual Macrophages with a Genetically-Encoded Probe.- The Conjugation of Antibodies for the Simultaneous Detection of Surface Proteins and Transcriptome Analysis at a Single-Cell Level.- Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling.- Proteomic Network Analysis of Polarized Macrophages.- The Purification and Characterization of Exosomes from Macrophages.- The Isolation and Characterization of T Lymphocyte Exosomes Using Mass Spectrometry.- Analysis of Immune-Tumor Cell Interactions Using a 3D Co-Culture Model.- Studying Adipocyte and Immune Cell Crosstalk Using a Co-Culture System.- A Gene Expression Analysis of M1 and M2 Polarized Macrophages.- Simultaneous, Quantitative Characterization of Protein ADP-Ribosylation and Protein Phosphorylation in Macrophages.- The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer.- Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by Assessing Their Oxygen Consumption and Extracellular Acidification.- The Evaluation of Mitochondrial Membrane Potential Using Fluorescent Dyes or a Membrane-Permeable Cation (TPP+) Electrode in Isolated Mitochondria and Intact Cells.- The Multiparametric Analysis of Mitochondrial Dynamics in T Cells from Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs).- The Measurement of Whole Body Glucose Homeostasis in Mice.- Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy.- Sex Differences in Immunometabolism: An Unexplored Area.- Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis.