Biophysics of Membrane Proteins

Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells.- Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-tag Labeling Approach.- Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes.- Functional Characterization of SLC transporters using Solid-Supported-Membranes.- Thermostability Assays Provide a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents.- Direct monitoring of GPCR reconstitution and ligand binding activity by Plasmon Waveguide Resonance.- Examining Membrane Proteins by Neutron Scattering.- Solution X-ray Scattering for Membrane Protein.- Interpreting SAXS/WAXS Data with Explicit-Solvent Simulations: A Practical Guide.- Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers.- Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry.- Determination of the Molecular Mass of Membrane Proteins Using Size Exclusion Chromatography with Multi-angle Laser Light Scattering (SEC-MALLS).- Dynamics of Membrane Proteins Monitored by Single-molecule Fluorescence across Multiple Time Scale.- Short-Range Distances Measurement by Transition Metal Ion FRET.- PELDOR/DEER – An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers.