Dynamin Superfamily GTPases

 Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae.- Purification of the Dynamin-related Protein Vps1 using Mammalian and Bacterial Expression Systems.- A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli.- Affinity Purification and Functional Characterization of Dynamin-related Protein 1.- Purification and Characterization of MxB.- Purification of Farnesylated hGBP1 and Characterization of Its Polymerization and Membrane Binding.- Microscale Thermophoresis (MST) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-lipid Interactions.- Nucleotide-dependent Dimerization and Conformational Switching of Atlastin.- Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System.- Cell-free Analysis of Mitochondrial Fusion by Fluorescence Microscopy.- Electrophysiological MethodsFor Detection Of Membrane Leakage and Hemifission by Dynamin 1.- Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore.- Cellular Assays for Measuring Dynamin Activity in Muscle Cells.- Measuring Drp1 Activity in Mitochondrial Fission in vivo.- Quantifying Drp1-mediated Mitochondrial Fission by Immunostaining in Fixed Cells.- Imaging Dynamin-related Protein 1 (Drp1)-mediated Mitochondrial Fission in Living Cells.