Leptospira spp.
Springer-Verlag New York Inc.
978-1-0716-0458-8 (ISBN)
Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.
Isolation and Culture of Leptospira from Clinical and Environmental Samples.- Core Genome Multi-Locus Sequence Typing Analyses of Leptospira spp. using the Bacterial Isolate Genome Sequence Database.- Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira.- Cell Enumeration of Leptospira by Flow Cytometry.- The Single-Step Method of RNA Purification Applied to Leptospira.- Purification of LPS from Leptospira.- Isolation, Purification, and Characterization of Leptophages.- Creating a Library of Random Transposon Mutants in Leptospira.- Transposon Sequencing in Leptospira interrogans.- Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically inactive Cas9 (dCas9).- Leptospira spp. Toolbox for Chemotaxis Assay.- In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography.- Measurement of the Cell-Body Rotation of Leptospira.- In Vivo Imaging of Bioluminescent Leptospires.- Cell Monolayer Translocation Assay.- Cell Adhesion Assay to Study Leptospiral Proteins: An Approach to Investigate Host-Pathogen Interaction.- Complement Resistance Assays.- Evaluation of Intracellular Trafficking in Macrophages.- Biofilm Formation and Quantification using the 96-Microtiter Plate.- Survival Tests for Leptospira spp..- Cultivation of Leptospira interrogans within Rat Peritoneal Dialysis Membrane Chambers.- Use of Golden Syrian Hamster as an Animal Model to Study Leptospirosis-Associated Immune Responses.- Evaluation of Vaccine Candidates against Leptospirosis using Golden Syrian Hamsters.- Leptospira and Leptospirosis.- Laboratory Diagnosis of Leptospirosis.
Erscheinungsdatum | 10.07.2020 |
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Reihe/Serie | Methods in Molecular Biology ; 2134 |
Zusatzinfo | 56 Illustrations, color; 10 Illustrations, black and white; X, 291 p. 66 illus., 56 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-0716-0458-9 / 1071604589 |
ISBN-13 | 978-1-0716-0458-8 / 9781071604588 |
Zustand | Neuware |
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