RNA Chaperones

Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones.- Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy.- Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity.- Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding.- Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein’s RNA Chaperone Activity.- Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function.- Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La.- Kinetic and Thermodynamic Analyses of RNA–Protein Interactions.- Detection of MicroRNAs Released from Argonautes.- Mapping the RNA Chaperone Activity of the T. brucei Editosome Using SHAPE-Chemical Probing.- RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing.- In Vivo RNA Chemical Footprinting Analysis in Archaea.- RNA Structure Analysis by Chemical Probing with DMS and CMCT.- Disordered RNA Binding Region Prediction with DisoRDPbind.- Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci.- Site-Specific Dual-Color Labeling of Long RNAs.- Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding.- Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy.- Isolation and Analysis of Bacterial Ribosomes through Sucrose Gradient Ultracentrifugation.