Advancing Gene Fusion Detection Towards Personalized Cancer Nanodiagnostics (eBook)

Supervisor’s Foreword 6
Parts of this thesis have been published in the following journal articles: 8
Acknowledgements 10
Contents 12
Abbreviations 14
1 Unifying Next-Generation Biomarkers and Nanodiagnostic Platforms for Precision Prostate Cancer Management 15
1.1 Introduction 15
1.2 Next-Generation PCa Biomarkers 18
1.3 Existing and Emerging Biomarker-Directed PCa Screening Diagnostics 22
1.4 Nano-strategies for Next-Generation PCa Biomarker Detection 27
1.5 Challenges in Clinical Translation of Novel Biomarkers and Nano-strategies 30
1.6 Conclusions and Future Outlook 33
References 34
2 Colorimetric Gene Fusion Diagnostics for Visual Binary Readout 44
2.1 Introduction 44
2.2 Materials and Methods 45
2.3 Results and Discussion 48
2.4 Conclusions 57
References 57
3 Label-Free Surface-Enhanced Raman Scattering Detection System for Clinical Biomarker Targets 58
3.1 Introduction 58
3.2 Materials and Methods 60
3.3 Results and Discussion 62
3.4 Conclusions 68
References 68
4 Amplification-Free Electrochemical RNA Biomarker Sensing 69
4.1 Introduction 69
4.2 Materials and Methods 71
4.3 Results and Discussion 73
4.4 Conclusions 80
References 81
5 Simultaneous Analysis of Multiple Biomarkers via High-Throughput Parallel Profiling 83
5.1 Introduction 83
5.2 Materials and Methods 84
5.3 Results and Discussion 87
5.4 Conclusions 92
References 94
6 Clinical Evaluation of Non-invasive Nanodiagnostics for PCa Risk Stratification 95
6.1 Introduction 95
6.2 Materials and Methods 97
6.3 Results and Discussion 99
6.4 Conclusions 108
References 109
7 Summary and Future Work 110
7.1 Summary 110
7.2 Future Work 110
Curriculum Vitae 112