Brain Tumor Stem Cells

Introduction to Brain Tumor Stem Cells.- Isolation and Culture of Glioblastoma Brain Tumor Stem Cells.- Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines.- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors.- Detecting Stem Cell Marker Expression Using the NanoString nCounter System.- Flow Cytometric Analysis of Brain Tumor Stem Cells.- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells.- Differentiation of Brain Tumor Initiating Cells.- The Study of Brain Tumor Stem Cell Migration.- The Study of Brain Tumor Stem Cell Invasion.- Cell Cycle Dynamics in Glioma Cancer Stem Cells.- Embryonic Stem Cell Models of Human Brain Tumors.- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory.- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF.- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells.- In Vitro Assays for Screening Small Molecules.- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma.- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci.- In Vivo Murine Models of Brain Metastasis.- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain.