MERS Coronavirus

PART I: Evolution and Entry of MERS-Coronavirus



1. Studying Evolutionary Adaptation of MERS-CoV



Michael Letko and Vincent Munster







2. Evaluating MERS-CoV Entry Pathways



Enya Qing, Michael P Hantak, Gautami G Galpalli, and Tom Gallagher







3. Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing



Gary R. Whittaker and Jean K. Millet







4. Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein



Haixia Zhou, Shuyuan Zhang, and Xinquan Wang







PART II: Genetic Alteration and Structural Determination of MERS-Coronavirus Proteins



5. Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV



Anthony R. Fehr







6. Deducing the Crystal Structure of MERS-CoV Helicase



Sheng Cui and Wei Hao







PART III: Quantitation of Virus and Anti-Viral Factors



7. Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans



Joshua Fung, Susanna K. P. Lau, and Patrick C. Y. Woo







8. Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR



Abdullah Algaissi, Anurodh S. Agrawal, Anwar M. Hashem, and Chien-Te K. Tseng







9. Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay Abdullah Algaissi and Anwar M Hashem







10. Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera Abdulrahman Almasaud, Naif Khalaf Alharbi, and Anwar M Hashem







11. Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA



Sawsan S Al-amri and Anwar M Hashem







PART IV: Mouse Models for MERS -Coronavirus



12. Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced



Acute Respiratory Distress Syndrome



Sarah R. Leist and Adam S. Cockrell







13. Development of a Mouse Adapted MERS Coronavirus



Kun Li and Paul B. McCray, Jr.







14. Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection



Carrie D. Nicora, Amy C. Sims, Kent J. Bloodsworth, Young-Mo Kim, Ronald J. Moore, Jennifer E. Kyle, Ernesto S. Nakayasu, and Thomas O. Metz







15. Evaluation of Activation and Inflammatory Activity of Myeloid Cells During



Pathogenic Human Coronavirus Infection



Rudragouda Channappanavar and Stanley Perlman



16. Histopathologic Evaluation and Scoring of Viral Lung Infection David K. Meyerholz and Amanda P. Beck