Protein Chromatography

Giorgio Carta, PhD, is a professor in the Department of Chemical Engineering at the University of Virginia, Charlottesville (USA), where his research focuses on transport phenomena and bioseparations. He regularly organizes professional courses on various aspects of bioseparations, including a course on protein chromatography development and scale-up together with Alois Jungbauer. Alois Jungbauer, PhD, is the head of protein technology and downstream processing at the Department of Biotechnology of the University of Natural Resources and Applied Life Sciences in Vienna (Austria). For more than 30 years, Professor Jungbauer has worked in biochemical engineering, with a focus on bioseparation, where he has published widely and holds 15 patents. He has also organized a biannual professional course in protein chromatography focused on mass transfer, dispersion, and scale-up.

Preface

DOWNSTREAM PROCESSING OF BIOTECHNOLOGY PRODUCTS
Introduction
Bioproducts and their Contaminants
Bioprocesses
Biosimilars
Role of Chromatography in Downstream Processing
Environmental Impact of Biopharmaceutical Processing
References

INTRODUCTION TO PROTEIN CHROMATOGRAPHY
Introduction
Basic Principles and Definitions
Modes of Operation
Performance Factors
Separation Performance Metrics

CHROMATOGRAPHY MEDIA
Introduction
Interaction Types and Chemistry
Buffers and Mobile Phases
Physical Structure and Properties

LABORATORY AND PROCESS COLUMNS AND EQUIPMENT
Introduction
Laboratory-scale Systems
Process Columns and Equipment

ADSORPTION EQUILIBRIUM
Introduction
Single Component Systems
Multi-component Systems
Empirical Correlation of Equilibrium Data
References

RATE PROCESSES
Introduction
Rate Mechanisms
Batch Adsorption Kinetics

DYNAMICS OF CHROMATOGRAPHY COLUMNS
Introduction
Material Balance Equations
Local Equilibrium Dynamics
Multi-component Systems
Displacement Chromatography

EFFECTS OF DISPERSION AND RATE PROCESSES ON COLUMN PERFORMANCE
Introduction
Empirical Characterization of Column Efficiency
Modeling and Prediction of Column Efficiency

GRADIENT ELUTION CHROMATOGRAPHY
Introduction
General Theory for Gradient Elution with Linear Isotherms
LGE Relationships and the Iso-Resolution for Ion Exchange Chromatography
LGE Relationships for RPC and HIC
Gradient Elution at High Protein Loads
Separations with pH Gradients
References

CHROMATOGRAPHIC COLUMN DESIGN ADN OPTIMIZATION
Introduction
Chromatographic Process Steps and Constraints
Design for Capture
Design for Chromatographic Resolution
SMB Design
References

INDEX