Microbial Proteomics

Filter-Aided Sample Preparation for Proteome Analysis.- Protein Enrichment from Highly Dilute Samples with StrataClean.- Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins.- Enrichment of Cell Surface-Associated Proteins in Gram+ Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis.- Preparation of Bacterial Magnetosomes for Proteome Analysis.- Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics.- Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS.- Applications of Difference Gel Electrophoresis (DIGE) on the Study of Microorganisms.- Proteomic Signatures in Staphylococcus aureus.- How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis.- Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE.- Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses.- Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N).- Next-Generation Trapping of Protease Substrates by Label-Free Proteomics.- In Vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host-Pathogen Settings.- Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography.- Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation.- Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria.- Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host-Microbiota Interactions.- Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis / Proteome Extraction from Soil Samples for Metaproteomics Studies.- Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter.- Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria-Invertebrate Symbioses.