Recombinant Protein Expression in Mammalian Cells

Transient Gene Expression in Suspension HEK293-EBNA1 Cells.- Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells.- PEI-Mediated Transient Gene Expression in CHO Cells.- Stable Expression by Lentiviral Transduction of Cells.- Inducible Protein Production in 293 Cells using the piggyBac Transposon System.- Recombinant CHO Cell Pool Generation using piggyBac Transposon System.- Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression.- Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor.- CHO and HEK293 Cell Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors.- Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary Cells in Suspension.- Continuous and Integrated Expression and Purification of Recombinant Antibodies.- High Throughput Transfection of HEK293 Cells for Transient ProteinProduction.- Microfluidic Transfection for High-Throughput Mammalian Protein Expression.- Genome Wide High Throughput RNAi Screening for Identification of Genes Involved in Protein Production.- Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells.- Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells.- Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV) .- Considerations in the Use of Codon Optimization for Recombinant Protein Expression.- Versatile Cell-free Protein Synthesis Systems based on Chinese Hamster Ovary Cells.