Biochemistry in the Lab - Benjamin F. Lasseter

Biochemistry in the Lab

A Manual for Undergraduates
Buch | Softcover
188 Seiten
2019
CRC Press (Verlag)
978-1-138-58996-4 (ISBN)
62,30 inkl. MwSt
Biochemistry in the Lab: A Manual for Undergraduates presents a comprehensive approach to modern biochemistry laboratory teaching together with a complete experimental experience, from molecular biology, cloning, and protein expression, to purification and characterization.
Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps.

Key Features:






Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience



Includes chemical biology as its foundation, teaching readers experimental methods specific to the field



Provides instructor experiments that are easy to prepare and execute, at comparatively low cost



Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry



Is written by an expert in the field

This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.

Dr. Benjamin F. Lasseter earned his Ph.D. in Biochemistry from Texas A&M University in 2003. He briefly assisted in the production of history textbooks for the Catholic Schools Textbook Project before teaching at a high school in Louisiana. He moved on to Christopher Newport University in 2011, where he teaches the biochemistry labs. He mixes analytical chemistry with pure biology to study environmental impacts of metal pollution.

Author Bio

Chapter 1 Buffers

Chapter 2 Assays

Chapter 3 Protein Concentration

Chapter 4 ELISA

Chapter 5 Salting Out Proteins and Other Biomolecules

Chapter 6 A Discussion of Isoelectric Point and Effective Charge

Chapter 7 Column Chromatography

Chapter 8 Michaelis–Menten Kinetics

Chapter 9 Protein Purification

Chapter 10 Polyacrylamide Gels

Chapter 11 In Silico Biochemistry: The Evolution of Globins

Chapter 12 Growing Crystals of Hemoglobin

Chapter 13 Enzyme Inhibition

Chapter 14 Multisubstrate Kinetics

Chapter 15 Fluorescence and Denaturation

Chapter 16 Fluorescence Studies of Ligand Binding

Chapter 17 DNA Restriction Digests

Chapter 18 Western Blotting

Index

Erscheinungsdatum
Zusatzinfo 41 Tables, black and white; 59 Illustrations, black and white
Verlagsort London
Sprache englisch
Maße 156 x 234 mm
Gewicht 303 g
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Chemie Analytische Chemie
Naturwissenschaften Chemie Organische Chemie
ISBN-10 1-138-58996-9 / 1138589969
ISBN-13 978-1-138-58996-4 / 9781138589964
Zustand Neuware
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