Plant Meiosis

A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis.- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes.- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana.- Cytological Characterization of Arabidopsis arenosa Polyploids by SIM.- Targeted Analysis of Chromatin Events (TACE).- Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH).- Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea.- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization.- Cytological Techniques to Study Cytomixis in Plant Male Meiosis.- Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn.- Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants.- Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids.- Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy.- Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium.- Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy.- Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers.- Isolating Male Meiocytes from Maize and Wheat for “-omics” Analyses.- How to Study the Proteomes and Phosphoproteomes of Anther and Pollen.- Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development.- Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei.- Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops.- A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS).- Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana.- Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species.- Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants.- A Modular Tray Growth System for Barley.- In planta Delivery of Chemical Compounds into Barley Meiocytes – EdU as Compound Example.